Development of an in vitro profiling platform for the mechanistic assessment of cytotoxic activities of (crotalid) snake venoms
Bittenbinder, Matyas A.
Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Faculty of Sciences, Vrije Universiteit Amsterdam, The Netherlands
Centre for Analytical Sciences Amsterdam (CASA), Amsterdam, The Netherlands
Naturalis Biodiversity Center, 2333 CR Leiden, The Netherlands
van der Wel, Nicole N.
Department Cell Biology and Histology, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands
Department Electron Microscopy Center Amsterdam, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, The Netherlands
Jennings, Paul
Division of Molecular Toxicology, AIMMS, Faculty of Sciences, Vrije Universiteit Amsterdam, The Netherlands
Kool, Jeroen
Centre for Analytical Sciences Amsterdam (CASA), Amsterdam, The Netherlands
Naturalis Biodiversity Center, Leiden, The Netherlands
Vonk, Freek J.
Division of BioAnalytical Chemistry, Department of Chemistry and Pharmaceutical Sciences, Amsterdam Institute of Molecular and Life Sciences (AIMMS), Faculty of Sciences, Vrije Universiteit Amsterdam, The Netherlands
Centre for Analytical Sciences Amsterdam (CASA), 1098 XH Amsterdam, The Netherlands
Naturalis Biodiversity Center, 2333 CR Leiden, The Netherlands
Snakebite is a global tropical disease that has long had huge implications for human health and well-being, with annual mortality ranging from 81,000 to 138,000. Although most life-threatening pathologies in snakebite victims result from hemotoxins and neurotoxins, cytotoxins are the main cause of life-long disabilities such as blindness and amputations. In order to better understand cytotoxic effects that venoms potentially can have on bite victims it is important to study the composition and biological function, both of crude venoms and their individual venom components. This research focuses on investigating methods to study mechanistic (molecular) mechanisms behind cytotoxicity including crotalid venoms. Varying activity patterns were observed for the panel of species used in this study. Additionally, MS data of the active components will be discussed in relation to the Viperidae and Elapidae. This research focused on investigating methods to study mechanistic (molecular) mechanisms behind cytotoxicity using 10 different medically relevant snake species. Combined, the integrated dataset provides us with an in vitro profiling platform that allows for the mechanistic assessment of cytotoxic activities of snake venoms.